Comprehensive ANA Profile (ANA + ENA Blot)

The Comprehensive ANA Profile (ANA + ENA Blot) is an advanced autoimmune test panel used to evaluate a wide range of autoimmune connective tissue diseases. It combines Anti-Nuclear Antibody (ANA) by Indirect Immunofluorescence Assay (IFA) with an Extractable Nuclear Antigen (ENA) Blot, which detects multiple disease-specific autoantibodies. This comprehensive profile helps investigate conditions such as systemic lupus erythematosus (SLE), Sjögren’s syndrome, systemic sclerosis, mixed connective tissue disease, inflammatory myopathies, and other autoimmune disorders, providing valuable support for accurate diagnosis and clinical assessment.

Who Should Get This Test Done?

This test may be recommended for individuals who:

●     Experience persistent joint pain, muscle weakness, fatigue, or unexplained fever suggestive of an autoimmune disorder.

●     Have symptoms such as dry eyes, dry mouth, skin thickening, or unexplained rashes.

●     Show a positive ANA screening result and require further evaluation with disease-specific antibodies.

●     Are being investigated for connective tissue diseases or systemic autoimmune conditions.

●     Need comprehensive autoimmune antibody testing as advised by a rheumatologist or healthcare provider.

Why Consider This Test?

The Comprehensive ANA Profile helps:

●     Screen for systemic autoimmune diseases with high sensitivity.

●     Identify disease-specific antibodies that aid accurate diagnosis.

●     Differentiate conditions such as lupus, Sjögren’s syndrome, systemic sclerosis, inflammatory myopathies, autoimmune liver diseases, and overlap syndromes.

●     Guide treatment decisions and disease monitoring.

●     Shorten the diagnostic journey, enabling earlier intervention and reducing the risk of irreversible organ damage.

More Information

This profile includes ANA by IFA, which evaluates the presence, staining pattern, and titre of antinuclear antibodies, along with an ENA Blot that detects multiple clinically significant autoantibodies, including Smith, U1 Sm/RNP, SSA (Ro), Ro-52, SSB (La), dsDNA, Histone, Centromere, Scl-70, PM-Scl, Jo-1, PCNA, Nucleosome, AMA-M2, Ribosomal P, Mi-2, Ku, and DFS70 antibodies. Assessing these markers together provides a comprehensive picture of autoimmune activity and helps differentiate between various connective tissue and systemic autoimmune diseases. The results are interpreted alongside the patient’s symptoms, medical history, and other diagnostic findings.

Other Names of the Test

●     Comprehensive ANA & ENA Profile

●     ANA with ENA Blot Panel

●     ANA Comprehensive Autoimmune Profile

●     Connective Tissue Disease Antibody Profile

●     ANA-IFA with ENA Autoantibody Panel